rodent derived p53 antibody  (Santa Cruz Biotechnology)

Journal: ACS Omega

Article Title: Injectable Thermosensitive Hydrogel Containing Resveratrol-Laden Nanoparticles Promotes Hypertrophic Scar Repair in Rat Tail

doi: 10.1021/acsomega.5c01469

Figure Lengend Snippet: Representative immunofluorescence-staining images of HIF-1α (A) and p53 (B) expression in scar tissue at week 0, week 2, and week 6 post-treatments. Scale bar = 200 or 50 μm. Quantitative evaluation of HIF-1α (C) and p53 (D) expression in scar tissue. Notes: compared with normal skin (NS), ** P < 0.01, *** P < 0.001,**** P < 0.0001; compared with NC, △△ P < 0.01, △△△△ P < 0.0001; compared with MSN@Res, # P < 0.05, ## P < 0.01, #### P < 0.0001.

Article Snippet: Subsequently, they were kept at 4 °C overnight in the presence of rabbit antiphosphorylated p38-MAPK (p-p38, bs-5476R, Bioss, China, 1:400), rabbit anti-LC3-II (LC3-II, 14600–1-AP, Aspen, China, 1:500), rabbit anti-cleaved caspase-3 (Cleaved Caspase 3, 25128–1-AP, Aspen, China, 1:150), rodent-derived p53 antibody (p53, 60283–2-IG, Aspen, China, 1:400), and a rat-originated HIF-1α antibody (HIF-1α, SC-13515, Santa Cruz, 1:100).

Techniques: Immunofluorescence, Staining, Expressing

Journal: Asian Pacific Journal of Tropical Biomedicine

Article Title: Hepatorenoprotective effects of Lepidium draba L. extracts against cyclophosphamide-induced oxidative injuries in rats via reducing apoptosis and inflammation

doi: 10.4103/apjtb.apjtb_518_24

Figure Lengend Snippet: Figure 6. Effects of L. draba extract on p53-positive cells in (A) kidney and liver tissues (DAB staining 400×, scale bar = 25 μm). (B) Quantitative results of immunohistochemical examination. Data are shown as mean ± SEM (n=6).

Article Snippet: The slides were then incubated in p53 rodent-specific monoclonal antibody (Cat. no. sc-98, Pab 1801, dilution 1꞉200, Santa Cruz Biotechnology) for 45 min at room temperature, then incubated for 5 min in 5% bovine serum albumin to block unbound antibodies, and finally in chromogen (3, 3’-diaminobenzidine) for 30 min at 37 曟. Counterstaining of the slides was performed with hematoxylin and 50 fields of view were randomly evaluated with a light microscope (Olympus CH3, Japan) attached to the camera (Moticam Technologies, Japan), and the p53 positive cells/total cells × 100 was calculated at 400× magnification and reported as mean (%) ± SEM[23].

Techniques: Staining, Immunohistochemical staining

Journal: Scientific Reports

Article Title: Regulation of cardiomyocyte DNA damage and cell death by the type 2A protein phosphatase regulatory protein alpha4

doi: 10.1038/s41598-021-85616-5

Figure Lengend Snippet: Regulation of the DNA damage response by α4 protein expression in cardiomyocytes. H9c2 cardiomyocytes were transfected with either 50 nM non-targeting control (NTC) siRNA or 50 nM siRNA specific to rat α4 protein (siα4) for 1–4 days (n = 4) and the KD of α4 protein expression was confirmed by Western analysis using an antibody raised against human α4 ( a ). α4 protein expression was knocked down for 4 days and Western analysis (short and long exposures) was used to probe samples (n = 3) with an antibody specific to phosphorylated S / T QG motifs within ATM/ATR substrates. Arrows indicate substrates whose phosphorylation status (n = 3) was elevated in response to α4 KD ( b ). The expression and phosphorylation (Ser15) of p53 were determined by Western analysis using rodent-specific antibodies raised against mouse p53 protein in samples following 4 days of α4 KD, n = 5 ( c ). The expression of PARP was determined by Western analysis (short and long exposures) following 1–4 days of α4 KD, n = 3. Arrows indicate full length PARP (116 kDa) and cleaved fragments (89 kDa and 24 kDa) ( d ). All data represents data mean values ± SEM and statistical significance vs. non-targeting control (NTC) was determined by a one-tailed Student’s T-test. Expression of β-actin was used to confirm equal protein loading between samples.

Article Snippet: Antibodies to detect Bax (#2772), Bcl-xL (#2762), phosphospecific ATM/ATR substrate (#6966), IGBP1 (α4) (#5699), PARP (#9542), phosphospecific (Ser139) H2AX (#9718), H2AX (#2595), rodent-specific p53 (#32532), rodent-specific phosphospecific (Ser15) p53 (#12571) and V5-tag (#13202) were obtained from Cell Signaling Technology (USA).

Techniques: Expressing, Transfection, Control, Western Blot, Phospho-proteomics, One-tailed Test